| Primary Identifier | MGI:5565343 | Allele Type | Targeted |
| Attribute String | Inducible, Null/knockout, Recombinase, Reporter, RMCE-ready | Gene | Entpd5 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| description | This exchange vector (along with the pDIRE plasmid containing an icre-expressioncassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-derived JM8.F6 knockout first reporter embryonic stem (ES) cells obtained from the Knockout Mouse Project (KOMP) consortium containing clone EPDO112_5_E02. Recombined ES cells, with the neo cassette removed and resistant to puro, were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony. |
| molecularNote | The allele was generated using a dual recombinase-mediated cassette exchange protocol. The exchange vector contained (from 5' to 3') an frt site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein (EGFP) seqe, a viral T2A oligopeptide, and a cre/ERT2 sequence. A rox-flanked puromycin resistance (puro) cassette and a loxP site were inserted at the 3' end of the exchange vector. |
