| Primary Identifier | MGI:5563563 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Mylk |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of the CArG (CC(A/T)6GG) regulatory element in exon 15. An FRT- and loxP-flanked neomycin resistance cassette was inserted downstream of the CArG element. Flp-mediated recombination removed the selection cassette and left the CArG element floxed. This allele targets the smMLCK isoform without affecting full-length Mylk or telokin. Western blot analysis confirmed normal expression of smMLCK isoform. |
