Primary Identifier | MGI:5563835 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Crb3 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An FRT-flanked neomycin resistance cassette was inserted downstream of exon 2. Exon 3 was replaced with a floxed exon 3-GFP fusion. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed the modified exon 3. Western blot analysis confirmed the absence of protein expression in E18.5 lungs and kidneys. |