Primary Identifier | MGI:5568246 | Allele Type | Targeted |
Attribute String | Conditional ready, Inducible, Recombinase | Gene | Hprt1 |
Transmission | Germline | Strain of Origin | B6N.Cg-A<w-J> Hprt1<b-m3> |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false | Project Collection | EUCOMMTOOLS |
molecularNote | The transgene was designed with an HS4 insulator region, the 2982 bp Ple266 minipromoter (derived from a portion of the endogenous promoter and one upstream putative regulatory element from the human S100 calcium binding protein B - S100B ), a chimeric intron cassette, the iCre/ERT2 fusion gene (with F3-frt sites flanking the ERT2 sequences), the mut6-WPRE element, an SV40 early polyA signal, an additional HS4 insulator region, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. |