| Primary Identifier | MGI:5568945 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Setd1b |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An FRT-flanked stop cassette containing a lacZ reporter, followed by loxP-flanked exon 5 and rox-flanked PGK-hygromycin cassette were inserted. Dre-recombination removed the PGK-hygromycin selection cassette. Flp mediated recombination removed the stop cassette leaving exon 5 floxed. Cre mediated recombination excised exon 5, resulting in frameshift. Western analysis confirmed lost of Setd1b protein. |
