| Primary Identifier | MGI:5571496 | Allele Type | Targeted |
| Attribute String | Modified isoform(s) | Gene | Lmna |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Introns 10 and 11 of Lmna were deleted thereby abolishing production of lamin C and producing only prelamin A. Exon 12 was modified such that the Mir9 seed-binding sequence in prelamin A's 3' UTR (CCAAAG) was modified to ACCCTG. A floxed neomycin resistance cassette was inserted downstream of the modified exon 12. Western blot analysis confirmed the increased expression of lamin A protein in cerebral cortex and the cerebellum. |
