| Primary Identifier | MGI:5575372 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready | Gene | Satb1 |
| Strain of Origin | (C57BL/6 x DBA/2)F1 x C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | TALEN based technology was used to insert the following construct into the target gene. A loxP followed by lox2272 was inserted upstream of exon2 and its start cocon. This was followed by an FRT flanked neomycin/kanamycin construct in reverse orientation. This was followed in turn by an EGFP/Satb1 3'UTR, again in reverse orientation. Finally, a second loxP followed by lox2272 was inserted. Cre expression will invert the sequence between loxP sites allowing expression of EGFP. |
