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Allele : Tg(Cck-cre,-lacZ)1Mini transgene insertion 1, Liliana Minichiello

Primary Identifier  MGI:5574585 Allele Type  Transgenic
Attribute String  Recombinase, Reporter Gene  Tg(Cck-cre,-lacZ)1Mini
Strain of Origin  (CBA x C57BL/6) Is Recombinase  true
Is Wild Type  false
molecularNote  A BAC (RP23-26B6) containing the Cck gene was modified by ET recombination so that a cassette composed of a hybrid intron, a cre recombinase gene, an internal ribosomal entry site (IRES), a beta-galactosidase gene (lacZ), and a neomycin/kanamycin resistance marker was inserted into the first ATG of the first coding exon (exon 2). The neo/kan marker was removed using flp recombinase. The purified construct was injected into the pronuclei of (CBA x C57BL/6) hybrid oocytes.
  • mutations:
  • Insertion
  • synonyms:
  • BAC-Cck-Cre,
  • BAC-Cck-Cre,
  • Cck-cre,
  • Cck-cre
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

0 Carried By

1 Driven By

5 Publication categories