| Primary Identifier | MGI:5574480 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Piezo2 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was constructed to insert loxP sites upstream of exon 43 and downstream of exon 45 of the Piezo2 gene. The vector contained an frt-flanked PGK-neo cassette upstream of the first loxP site. Following homologous recombination in ES cells, the PGK-neo marker and a single loxP site were upstream of exon 43 and a second loxP site was downstream of exon 45.Chimeric animals were crossed to EIIa-cre expressing mice to delete exons 43-45, causing a frameshift that results in production of a premature stop codon. |
