| First Author | Wang Y | Year | 2020 |
| Journal | STAR Protoc | Volume | 1 |
| Issue | 3 | Pages | 100171 |
| PubMed ID | 33377065 | Mgi Jnum | J:354758 |
| Mgi Id | MGI:7736448 | Doi | 10.1016/j.xpro.2020.100171 |
| Citation | Wang Y, et al. (2020) Protocol for Monitoring DNA-Triggered cGAS/STING Signaling in Mammalian Cells and Mice. STAR Protoc 1(3):100171 |
| abstractText | Innate immunity is the first layer of defense against infection in mammals and is tightly regulated. We monitored cGAS/STING signaling upon ISD90 or 2',3'-cGAMP stimulation in EA.hy926 cells by western blotting, RT-PCR, and ELISA analyses to reveal signaling activation and IFNbeta production. In addition, we also include an HSV-1 infected mouse model to further reveal procedures in analyzing cGAS/STING signaling in mice. This protocol could be applied to studies focusing on cell culture or mouse models to investigate cGAS/STING signaling. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020). |
