Primary Identifier | MGI:5576462 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Slc1a2 |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6J)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted into intron 2 and an frt-neo-frt-loxP cassette was inserted into intron 4, floxing exons 3 and 4 of the Slc1a2 gene from the bacterial artificial chromosome clone RP24-315H4. The neomycin cassette was removed via FLP-mediated recombination, leaving exons 3 and 4 floxed by loxP sites. Cre-mediated recombination can be used to remove exons 3 and 4 which results in a frameshift deletion. |