| Primary Identifier | MGI:5578554 | Allele Type | Transgenic |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Tg(DMD)72Thoen |
| Transmission | Germline | Strain of Origin | (DBA/2 x 129P2/OlaHsd)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The construct consists of a 2.7 Mbp yeast artificial chromosome (YAC) sequence containing the full-length 2.3 Mbp human dystrophin gene (DMD) and a hygromycin resistance cassette on one end and a neomycin resistance cassette on the other. Spheroplast fusion of YAC-containing yeast cells with murine (DBA2 x 129OLA)F1-derived embryonic stem (ES) cells resulted in ES cells with the integration of the transgene as a tail to tail duplication into mouse chromosome 5. Analysis by Western blot and human-specific antibody demonstrated the presence of full length protein at the sarcolemma. |
