| Primary Identifier | MGI:5578604 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mgat4b |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of exons 2, 3, and 4, and a loxP site upstream of the exons. Cre-mediated recombination removed the neo cassette and exons 2-4 leaving a residual loxP site. Removal of exons 2-4 causes a frameshift at the junction of exon 1 and 5, generating a new translational termination codon that results in a truncated protein lacking the catalytic domain. |
