Primary Identifier | MGI:5585716 | Allele Type | Targeted |
Attribute String | Inducible, Inserted expressed sequence, Reporter, RMCE-ready | Gene | Col1a1 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Induced With | doxycycline/tetracycline | Is Recombinase | false |
Is Wild Type | false |
description | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae (J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae. |
molecularNote | KH2 ES cells were retargeted with a "flp-in plasmid containing a tetracycline responsive element (TRE or tetO), FLAG-tagged Fosl1 cDNA sequence, GtX IRES sequence and DsRed reporter. A flpe-recombinase plasmid facilitated integration into the 3' UTR region. |