| Primary Identifier | MGI:5584282 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Gcm2 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector contains a FRT-flanked neomycin selection cassette and a loxP site inserted downstream of exon 2 of the targeted gene, and another loxP site inserted upstream of exon 2. The neomycin cassette was deleted by transient transfection using flp recombinase. Cre-mediated recombination removed exon 2. The deletion creates a frameshift followed by a stop codon that results in the production of a short peptide lacking the main functional domains of the endogenous gene. |
