| Primary Identifier | MGI:5585450 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mir22 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 2 was flanked by loxP sites and a positive-selection neomycin cassette flanked by FRT sites was inserted downstream of exon 2. The neomycin cassette was excised by FLP-mediated recombination. The floxed exon 2 from Mir22tm1.1Dzw was removed via Cre-mediated recombination leaving a single loxP in place of exon 2. Northern blot and quantitative real-time PCR analysis confirmed absence of expression. |
