| Primary Identifier | MGI:5586533 | Allele Type | Targeted |
| Attribute String | Inducible, Inserted expressed sequence, RMCE-ready | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Induced With | doxycycline/tetracycline | Is Recombinase | false |
| Is Wild Type | false |
| description | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae (J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae. |
| molecularNote | KH2 ES cells were retargeted with a "flp-in plasmid" containing a tetracycline responsive element (TRE or tetO) and a forced dimerization fusion cDNA of Jun lacking the stop codon and Fosl2 connected by a flexible short polyGly-Ser peptide with a C-terminal FLAG-tag. A flpe-recombinase plasmid facilitated integration into the 3' UTR region. |
