Primary Identifier | MGI:5582820 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Nkx2-5 |
Transmission | Germline | Strain of Origin | 129X1/SvJ |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to insert a single loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, upstream of exon 2 of the gene. A 3X Flag sequence and a second loxP site were inserted into exon 2, upstream of the Nkx2-5 stop codon. FLP mediated recombination removed the FRT-flanked neo cassette. |