| Primary Identifier | MGI:5588813 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Tshz1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A GAP43-EGFP cassette and a self-excision cre-neomycin cassette (SEcre Neo) was fused in-frame following the first five codon of exon2 while removing most of the open reading frame of Tshz1 gene by homologous recombination. SEcre Neo cassette was removed in chimera male germline leaving fusion protein contains the first 18 amino acids of TSHZ1 (ending in YVPE encoded at the start of exon 2), followed by the N-terminal 23 amino acids of GAP43 and EGFP sequences, ending with the termination codon of EGFP. The in situ hybridization and immunostaining on homozygous embryos confirmed absence of transcripts and functional protein products. This is a null allele. |
