| Primary Identifier | MGI:5603434 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Fezf1 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Induced With | trimethoprim | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | ES cells previously targeted with a T2A-Cre vector immediately downstream of the Fez family zinc finger 1 translational STOP codon were re-targeted with a "T2A-hDHFR/Cre" vector and a FLP-expressing plasmid to facilitate recombination. Correctly re-targeted ES cells had (from 5' to 3') a partial Fezf1 intron 3 sequence containing an frt3 site, a partial Fezf1 exon 4 sequence up to (but not including) the endogenous stop codon, a T2A cleavage sequence that is in-frame with the Fezf1 coding sequence, a dCre fusion gene (described below) that is in-frame with the Fezf1 coding sequence, a bovine growth hormone polyA sequence, an AttB sequence, a PGK-hygromycin-SV40polyA cassette (with an mRNA spice donor-frt5 site-mRNA spice acceptor in the hygromycin gene), and an AttP sequence. The dCre fusion gene (also called destabilized Cre, DHFR/Cre or ecDHFR |
