| Primary Identifier | MGI:5606046 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Null/knockout | Gene | Ryr2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to replace exon 3 with a deletion of the 105 bp exon 3 sequence and the intron sequences (15 bp on both sides) that flank exon 3 and a FRT flanked neomycin cassette. The neomycin cassette was removed via Flp-mediated recombination. Western blot analysis of heterozygotes indicates that protein level in hearts is reduced to 58% of wild-type hearts, indicating impaired expression of this allele. |
