| Primary Identifier | MGI:5607579 | Allele Type | Targeted |
| Attribute String | Conditional ready, Reporter | Gene | Mpl |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An IRES-GFP-FRT-PGK-neo-FRT-loxP cassette was inserted immediately downstream of the stop codon in exon 12, along with a loxP site in intron 10. The PGK-neo cassette was removed via Flp-mediated recombination, leaving exons 11-12 and the IRES-GFP floxed. Expression of GFP was confirmed by flow cytometry and live cell imaging in megakaryocyte progenitor cells, megakaryoctyes, and LSK cells. |
