| Primary Identifier | MGI:5616091 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Entpd3 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The start codon, located in exon 2, was replaced with an AscI site to facilitate cloning of AscI-LoxP-EGFPf-3xpA-LoxP-DTR-pA-Frt-PGK-NeoR-Frt-AscI. Flp-mediated recombination removed the FRT-flanked selection cassette. Immunoblot analysis confirmed the absence of protein in dorsal root ganglion (DRG), spinal cord, hindpaw skin, and bladder tissue from adult homozygous mutant mice. Note: the knocked-in GFP was undetectable in mutant DRG and spinal cord neurons. |
