| First Author | Thomasi B | Year | 2026 |
| Journal | Methods Mol Biol | Volume | 2971 |
| Pages | 121-133 | PubMed ID | 41028630 |
| Mgi Jnum | J:390529 | Mgi Id | MGI:8398353 |
| Doi | 10.1007/978-1-0716-4795-0_12 | Citation | Thomasi B, et al. (2026) Calcium Imaging Studies in Enteric Glia. Methods Mol Biol 2971:121-133 |
| abstractText | Calcium (Ca(2+)) imaging is a versatile technique to study cellular activity and is a particularly important tool to study electrically passive cells such as glia. Enteric glia play important roles in gut physiology, and their activity can be captured using Ca(2+) imaging. Ca(2+)-encoded activities command glial processes such as gliotransmission and intercellular signaling. This chapter describes a protocol that will allow users to study glial Ca(2+) activity in ex vivo whole-mounts of circular muscle-myenteric plexus. This chapter will include comprehensive steps to execute Ca(2+) imaging experiments, data extraction, and analysis. |
