| Primary Identifier | MGI:5636788 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Shox2 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A Flag-HA-Shox2a-DsRed-polyA cassette and an FRT flanked neo cassette were inserted at the start site in exon 1 via homologous recombination. Flp mediated recombination removed the neo cassette. The Shox2a cDNA encodes an N-terminal FLAG-HA- tagged full length Shox2 gene. The Shox2a cDNA has a heterologous 3'UTR releasing the gene from endogenous miRNA control. Immunofluorescence analysis indicates aberrant elevated expression of the mutant protein at E16.5 in the atrial septum. |
