| Primary Identifier | MGI:5636740 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Spag17 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Phenotypic Similarity to Human Syndrome: Primary Ciliary Dyskinesia in Spag17tm1d(KOMP)Wtsi homozygous mice (J:211752) |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 99889093 of Chromosome 3 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 99889997. Exon 5 is thus flanked by loxP sites. Flp and cre mediated recombination removed the cassette and exon 5. Western blotting showed absence of the encoded protein from tracheal tissue extracts, and immunofluorescence and immunocytochemistry studies confirmed the absence of protein in tracheal epithelial cells and brain ependymal cells from homozygous mutant mice. |
