| Primary Identifier | MGI:5637591 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cacna1i |
| Inheritance Mode | Recessive | Transmission | Germline |
| Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A 6.8-kb genomic DNA segment containing exons 11-21 has been replaced with a cassette containing a EN2-SA-internal ribosomal entry site (IRES) followed by a beta-galactosidase-neomycin phosphotransferase (-neo) fusion gene (betageo). A loxP-flanked selection cassette containing Pgk-neo and MC1 tk genes was deleted by cre recombinase. The deleted segment encodes the predicted IIS2 and IIS4 transmembrane domains and the third predicted intracellular domain; the modification also is predicted to cause a frame-shift upon splicing of exon 10 to exon 22. RT-PCR analysis of RNA from cerebellum, cortex, olfactory bulb, and thalamus of homozygous mutant mice confirmed that no full-length Cacna1i mRNA is generated. |
