Primary Identifier | MGI:5638555 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Magoh |
Transmission | Germline | Strain of Origin | C57BL/6J |
Is Recombinase | false | Is Wild Type | false |
description | J:213515 used the KOMP vector PRPGS00072_A_G06 to create this allele. |
molecularNote | The L1L2_Bact_P cassette was inserted at position 107737628 of Chromosome 4 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 107738438. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp mediated recombination. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. |