Primary Identifier | MGI:5638895 | Allele Type | Targeted |
Attribute String | Conditional ready, Inserted expressed sequence, Null/knockout | Gene | Ewsr1 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP-flanked transcriptional stop signal in the antisense direction was inserted into intron 6. Exon 7 was fused to exons 8-10 of human WT1 and a 3' neo cassette was inserted, replacing exons 8 through 10 by homologous recombination. The absence of endogenous protein product was confirmed by western blot analysis. |