| Primary Identifier | MGI:5638905 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae (J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae. |
| molecularNote | RMCE on KH2 ES cells introduced into the 3' UTR the Sry coding region with 269 bp of the upstream flanking inverted repeat and downstream inverted repeat up to but not including the StuI restriction site followed by an ampillicin resistance gene, PGK promoter, hygromycin resistance gene and polyadenylation sequence. FRT sites flank the entire insertion. |
