| Primary Identifier | MGI:5644468 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Chn1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele from project Chn1-6579J-M1939 was generated at The Jackson Laboratory by injecting Cas9 RNA and 2 guide sequences, TTTAAGCAGTCTCGGTGAAA and CCAAGGACATCAGCTCTTGG, (along with a plasmid containing 1 kb homology arms flanking the floxed critical exon which did not integrate) which resulted in a 403 bp deletion and a 12 bp insertion of ttaagccaagtc in exon3 beginning at Chromosome 2 negative strand position 73,716,700 bp, GAAAAGGTTGTTTAATCACCA, and ending after TCCCAAGAGCTGATGTCCTT at 73,716,298 bp (GRCm38/mm10). This mutation deletes exon3 and is predicted to cause amino acid sequence changes after residue 19 and early truncation 17 amino acids later. PCR failed to detect the insertion of any loxP sites. |
