| Primary Identifier | MGI:5645738 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Per3 |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting construct contained a cDNA sequence containing sequences of wild-type (WT) mouse exons 1821, with a downstream phosphoglycerine kinase (PGK) promoter driving a neomycin (Neo) selection marker. The sequence was flanked by locus of loxP sites and followed by the human exon 18, containing the 5-repeat allele of the human variable-number tandem-repeat (VNTR). Cre mediated recombination removed the cDNA encoding sequences contained in exons 1821, allowing the expression of humanized exon 18 with the 5-repeat copy of the human variable-number tandem-repeat (VNTR) and endogenous mouse exons 19-21. |
