| Primary Identifier | MGI:5645820 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | F13b |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line EB3/5 is a mutant E14TG2a line carrying one inactivated allele of Pou5f1. J:222849 does not specify whether the line of mice created carries the inactivated Pou5f1 allele. |
| molecularNote | A genomic fragment including all of exons 1 and 2 was replaced with a neo cassette via homologous recombination. Northern and western blot analysis confirmed the absence of mRNA and protein expression in homozygous mice. Expression of F13a1 was greatly reduced compared to wild-type in homozygous mice. |
