| First Author | Singh U | Year | 2022 |
| Journal | Mol Metab | Volume | 55 |
| Pages | 101401 | PubMed ID | 34823066 |
| Mgi Jnum | J:330365 | Mgi Id | MGI:6842746 |
| Doi | 10.1016/j.molmet.2021.101401 | Citation | Singh U, et al. (2021) Neuroanatomical organization and functional roles of PVN MC4R pathways in physiological and behavioral regulations. Mol Metab 55:101401 |
| abstractText | OBJECTIVE: The paraventricular nucleus of hypothalamus (PVN), an integrative center in the brain, orchestrates a wide range of physiological and behavioral responses. While the PVN melanocortin 4 receptor (MC4R) signaling (PVN(MC4R+)) is involved in feeding regulation, the neuroanatomical organization of PVN(MC4R+) connectivity and its role in other physiological regulations are incompletely understood. Here we aimed to better characterize the input-output organization of PVN(MC4R+) neurons and test their physiological functions beyond feeding. METHODS: Using a combination of viral tools, we mapped PVN(MC4R+) circuits and tested the effects of chemogenetic activation of PVN(MC4R+) neurons on thermoregulation, cardiovascular control, and other behavioral responses beyond feeding. RESULTS: We found that PVN(MC4R+) neurons innervate many different brain regions that are known to be important not only for feeding but also for neuroendocrine and autonomic control of thermoregulation and cardiovascular function, including but not limited to the preoptic area, median eminence, parabrachial nucleus, pre-locus coeruleus, nucleus of solitary tract, ventrolateral medulla, and thoracic spinal cord. Contrary to these broad efferent projections, PVN(MC4R+) neurons receive monosynaptic inputs mainly from other hypothalamic nuclei (preoptic area, arcuate and dorsomedial hypothalamic nuclei, supraoptic nucleus, and premammillary nucleus), the circumventricular organs (subfornical organ and vascular organ of lamina terminalis), the bed nucleus of stria terminalis, and the parabrachial nucleus. Consistent with their broad efferent projections, chemogenetic activation of PVN(MC4R+) neurons not only suppressed feeding but also led to an apparent increase in heart rate, blood pressure, and brown adipose tissue temperature. These physiological changes accompanied acute transient hyperactivity followed by hypoactivity and resting-like behavior. CONCLUSIONS: Our results elucidate the neuroanatomical organization of PVN(MC4R+) circuits and shed new light on the roles of PVN(MC4R+) pathways in autonomic control of thermoregulation, cardiovascular function, and biphasic behavioral activation. |
