| Primary Identifier | MGI:5648555 | Allele Type | Transgenic |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Tg(Igf2/LacZ,H19)YZ17Aco |
| Strain of Origin | (C57BL/6 x CBA)F2 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The 130 kb YAC transgene, YZ, contains the Igf2 and H19 genomic regions and in which the Igf2 coding sequence was interrupted with the lacZ reporter gene. The YZ YAC extends from promoter 1 of Igf2 to 35 kb downstream of H19, and includes the differentially methylated region around exons 5 to 6 of Igf2, the unmethylated intergenic hypersensitive region, A6-A4, the differentially methylated regions around H19 and the endoderm-specific enhancers downstream of H19. In addition, the 1kb conserved intergenic region is flanked with LoxP sites. LacZ activity shows spatial expression of Igf2 in a subset of tissues. The imprinted H19 gene is only expressed when transmitted through the maternal germline. After paternal transmission the gene is repressed. Line YZ17 carries 2 copies of the transgene. |
