| Primary Identifier | MGI:5662395 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase, Reporter | Gene | Tfcp2l1 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The targeting construct was generated by inserting the diphtheria toxin A gene (DTA, for positive selection), 3.2 kb 5 homologous arm containing the 5' UTR of exon 1, and 5.5 kb 3 homologous arm containing exon 2 into pBluescript SKII(+). Then the eGFP-CreERT2 (GCE) fragment (McMahon et al. 2008) with an SV40 poly adenylation site and neomycin resistance cassette were inserted immediately downstream of the translational initiation codon ATG. The knock-in construct removed the coding sequences from exon 1 and placed the GCE gene under the control of endogenous regulatory sequences. Flp-mediated recombination removed the selection cassette. The EGFP could not be detected by direct fluorescence or anti-GFP antibody staining. |
