Primary Identifier | MGI:5688572 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Itgb3 |
Transmission | Germline | Strain of Origin | C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley |
Is Recombinase | false | Is Wild Type | false |
description | B6/BLU embryonic stem cells contain a LacZ reporter fused to beta-globin and is expressed in peripheral blood cells. |
molecularNote | The targeting vector was designed to insert a single loxP site upstream of exon 1 and loxP-flanked PGK-neomycin resistance (neo) cassette downstream of exon 1. The neo cassette was deleted by transiently transfection of a pTURBO-Cre expression plasmid leaving exon 1 floxed. |