| Primary Identifier | MGI:5689439 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Del(16Kcnj15-Mx2)13Yey |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | hromosome-engineering cassettes were inserted into mouse Chromosome 16 to bracket a span between the potassium inwardly-rectifying channel, subfamily J, member 15 (Kcnj15) gene and the MX dynamin-like GTPase 2 (Mx2) gene. The cassette placed at the proximal locus (MICER clone MHPP54c08) was targeted upstream of Kcnj15 and contained a neomycin resistance gene, a loxP site, a 5' portion of an HPRT minigene, and a tyrosinase minigene. The cassette placed at the distal locus (MICER clone MHPN178b08) was targeted downstream of the Mx2 gene and contained an agouti transgene, a 3' portion of an HPRT minigene, a loxP site and puromycin resistance gene. Double-targeted ES cells were transiently transfected with the cre recombinase expression vector pOG231 to remove the region from Kcnj15 to Mx2, which encompasses 16 genes. FISH analysis of ES cell clones confirmed the deletions. |
