| Primary Identifier | MGI:5689441 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Del(16Dyrk1a-Kcnj6)14Yey |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Chromosome-engineering cassettes were inserted into mouse Chromosome 16 to bracket a span between the dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a) gene and the potassium inwardly-rectifying channel, subfamily J, member 6 (Kcnj6) gene. The cassette placed at the proximal locus (MICER clone MHPN85f19) was targeted upstream of Dyrk1a and contained a neomycin resistance gene, a loxP site, a 5' portion of an HPRT minigene, and a tyrosinase minigene. The cassette placed at the distal locus (MICER clone MHPP54c08) was targeted downstream of the Kcnj6 gene and contained an agouti transgene, a 3' portion of an HPRT minigene, a loxP site and puromycin resistance gene. Double-targeted ES cells were transiently transfected with the cre recombinase expression vector pOG231 to remove Dyrk1a and Kcnj6. FISH analysis of ES cell clones confirmed the deletions. |
