Primary Identifier | MGI:5688642 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Enho |
Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A neo cassette was inserted in the 5' region of exon 2 and 126 bp including a loxP site was inserted in the 3' region via homologous recombination. RT-PCR detected no change in mRNA expression of Enho or Dnaic1. Western blot analysis confirmed the absence of ENHO protein expression. |