| Primary Identifier | MGI:5697198 | Allele Type | Transgenic |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Tg(ATXN2*72Q)#Plt |
| Strain of Origin | FVB/N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A 169 kb human BAC (RP11-798L5) containing the entire 150 kb human ATXN2 locus including regulatory regions was engineered to replace the endogenous ATXN2 exon-1 CAG22 with CAG72 repeats. Quantitative PCR analysis indicates tandem integration of 4 copies of the transgene. RT-PCR confirmed expression of transcripts throughout the central nervous system, including cerebral hemispheres, cerebellum and spinal cord, and in non-central nervous system tissues like the heart and liver. Western blot confirmed expression of protein. The pound symbol (#) is used when no line is specified and/or lines are pooled. |
