| Primary Identifier | MGI:5698367 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Scube2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Cre-mediated recombination of Scube2tm1Rbya resulted in a 65kb deletion encompassing exons 2-21 of the gene. The deleted region contains the coding sequences for nine tandem epidermal growth factor-like domains, the spacer region, three cysteine-rich repeats, and the CUB domain of the encoded protein. The deletion also introduces a reading frame shift. RT-PCR with primer pairs specific for the wild-type mRNA detected the transcript in MEFs and in multiple tissues, at the highest levels in heart, kidney and testis, of wild-type mice, but no expression in any tissue analyzed from homozygous mutant mice. This was confirmed for the protein in heart, lung and testis by western blot analysis. |
