| Primary Identifier | MGI:5698528 | Allele Type | Targeted |
| Attribute String | Constitutively active | Gene | Lhx1 |
| Transmission | Germline | Strain of Origin | 129S/SvEv-Gpi1<c> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Using vector PGRS0002_B_E08, the L1L2_Bact_P cassette was inserted at position 84522718 of Chromosome 11 upstream of the critical exons 2 and 3 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 and 3 at position 84521461. The critical exons 2 and 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. |
