Primary Identifier | MGI:5700398 | Allele Type | Targeted |
Attribute String | Conditional ready, Recombinase | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | true | Is Wild Type | false |
molecularNote | The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, a flpo recombinase gene (a codon-optimized flpe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), and a Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. |