| Primary Identifier | MGI:5700416 | Allele Type | Targeted |
| Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, an FRT-flanked tdTomato sequence (tdTomato is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), three copies of green fluorescent protein, and a Neo-polyA cassette. The three GFP sequences include emerald, TagGFP (Aequorea macrodactyla GFP), and hrGFP (Humanized Renilla reniformis GFP) variants. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. |
