| Primary Identifier | MGI:5702728 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change, Reporter | Gene | Exosc3 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac or C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| description | "The...targeting vector was electroporated into ROSA26CreERt2/+, 129S6/SvEvTac x C57BL/6Tac hybrid ES cells." (J:217084) |
| molecularNote | Lox2372 and loxP sites were inserted in intron 1 and an antisense oriented conditional inversion (COIN) module, a second set of lox2372 and loxP sites, and an FRT flanked neo cassette were inserted in intron 3 The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the intervening sequence. The COIN module consists of a splice acceptor site and a T2A-GFP-pA cassette. Flp-mediated recombination removed the drug selection cassette. |
