| Primary Identifier | MGI:5705846 | Allele Type | Targeted |
| Gene | Fermt3 | Transmission | Germline |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Site-directed mutagenesis mutated CAATGG into GCCGCC in exon 14, which led to a substitution of Q<597>W<598>/AA. An FRT-flanked neomycin cassette in intron 13 was removed via Flp-mediated recombination. Western blot analysis revealed normal expression levels of encoded protein in washed platelets from mutant mice. Coimmunoprecipitation experiments revealed a ~85% reduction in the association between integrin alphaIIbbeta3 and mutant protein in platelet lysates. |
