| Primary Identifier | MGI:5705748 | Allele Type | Transgenic |
| Attribute String | Humanized sequence, Inserted expressed sequence, Reporter | Gene | Tg(OPTN*E50K,-EGFP)1Mde |
| Strain of Origin | (C57BL/6 x SJL)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The transgene is composed of BAC clone RP11-1107F3 containing the 38 kb-human OPTN locus with about 160 kb of 5 sequence containing the E50K mutation in the middle (GAG to AAG) and a fragment containing the internal ribosome entry site-enhanced green fluorescent protein. Two lines were established, 1 and 2. Line 1 shows overexpression of the gene but line 2 does not. Quantitative rtPCR and immunoblot analysis confirmed mRNA expression and protein expression in the retina. |
