| Primary Identifier | MGI:5708408 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Igh |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A construct containing an unmutated version of VDJH2 from the 8D10 hybridoma (VGAM3.8 family VGK7 germline gene rearranged to D and JH2) with a TGT to TGC mutation at codon 92 to remove a cryptic heptamer site was inserted in the JH4 intron. To create the "unmutated" VDHJ2 a 123 bp region containing the mutated Vh region was replaced with the corresponding piece from a VGK7 germline gene. A floxed neo selection cassette was removed by cre mediated recombination. |
