| First Author | Rickgauer JP | Year | 2014 |
| Journal | Nat Neurosci | Volume | 17 |
| Issue | 12 | Pages | 1816-24 |
| PubMed ID | 25402854 | Mgi Jnum | J:228932 |
| Mgi Id | MGI:5749794 | Doi | 10.1038/nn.3866 |
| Citation | Rickgauer JP, et al. (2014) Simultaneous cellular-resolution optical perturbation and imaging of place cell firing fields. Nat Neurosci 17(12):1816-24 |
| abstractText | Linking neural microcircuit function to emergent properties of the mammalian brain requires fine-scale manipulation and measurement of neural activity during behavior, where each neuron's coding and dynamics can be characterized. We developed an optical method for simultaneous cellular-resolution stimulation and large-scale recording of neuronal activity in behaving mice. Dual-wavelength two-photon excitation allowed largely independent functional imaging with a green fluorescent calcium sensor (GCaMP3, lambda = 920 +/- 6 nm) and single-neuron photostimulation with a red-shifted optogenetic probe (C1V1, lambda = 1,064 +/- 6 nm) in neurons coexpressing the two proteins. We manipulated task-modulated activity in individual hippocampal CA1 place cells during spatial navigation in a virtual reality environment, mimicking natural place-field activity, or 'biasing', to reveal subthreshold dynamics. Notably, manipulating single place-cell activity also affected activity in small groups of other place cells that were active around the same time in the task, suggesting a functional role for local place cell interactions in shaping firing fields. |
